I-Nucleic Acid Extraction okanye iKhiti yokuCoca
Nucleic Acid Eukutsalwa Or Ukusulungekiswa Kitokanye igcinwe ku -20℃. Isampulu kufuneka ithuthwe kusetyenziswa i-0℃ curling.
Introduction
I-Nucleic Acid Extraction okanye i-Curification Kit (iNdlela ye-Magnetic Beads) yenzelwe ukuhlanjululwa ngokuzenzekelayo kwe-RNA kunye ne-DNA kumanzi omzimba (njenge-swabs, i-plasma, i-serum) usebenzisa izixhobo ze-automated nucleic acid extraction. Itekhnoloji ye-Magnetic-particle ibonelela nge-DNA/RNA ekumgangatho ophezulu elungele ukusetyenziswa ngokuthe ngqo kwizicelo ezisezantsi ezifana nokukhulisa okanye ezinye ii-enzymatic reactions.
Application Range
Igazi elipheleleyo, i-plasma, i-serum kunye nezinye iisampulu zethishu ziye zahlanjululwa ngokuthe ngqo kwaye zetyiswa. I-nucleic acid ekhutshiweyo iye yakhethwa ngokukhethekileyo ngamaso emagnetic nanometer amakhulu. Emva koko iprotheni, i-ion yetyuwa ye-inorganic kunye nokungcola kwezinto eziphilayo zasuswa ngokuhlamba isisombululo. Ekugqibeleni, i-nucleic acid yachithwa kunye ne-eluent ukufumana isisombululo esicocekileyo se-nucleic acid.
Kit Contents
| Ikati. Hayi. | I-YXN-VIRAL01-32A-BR | Amacandelo | ||
| -50A | - 100A | |||
| Ubungakanani | 32Tes | 50Uvavanyo | 100Vavanyo | |
| ULysis Buffer | 96 wellPre-pack ed Amacwecwe Iziqwenga ezi-2 | 25ml | 50ml | Surfactant kunye Tris |
| Hlamba iBuffer I | ★15ml | ★30ml | Isisombululo setyuwa eninzi | |
| Hlamba i-Buffer Il | ★6ml*2 | ★12ml*2 | Isisombululo setyuwa ephantsi | |
| Elution Buffer | 10ml | 20ml | Isisombululo setyuwa ephantsi | |
| MagaBio Reagent | 1.0ml | 2.0ml | Amasuntswana emagnethi | |
| Incwadi yesandla(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Namanqaku:KubaI-YXN-VIRAL01-32A-BR-50A,yongeza 15mL Absolute ethanol ukuya ★15mL Hlamba iBuffer I phambi kokuba uyisebenzise; yongeza 24mL Absolute ethanol ukuya ★6mL Hlamba isithinteli Il phambi kokuba uyisebenzise. | ||||
| KubaYXN-VIRAL01-32A-BR-100A, yongeza 30mL Absolute ethanol kwi ★30mL Hlamba iBuffer I phambi kokuba uyisebenzise; yongeza 48mL Absolute ethanol ku ★12mL Hlamba isithinteli Il phambi kokuba uyisebenzise.【Iizinto ezisebenza ngokutsha mazilungiswe ngumsebenzisi】Nceda ulungise i-ethanol epheleleyo (ibakala lohlalutyo) wedwa. | ||||
Sti-oreji Condinto mnaons
Ekufikeni kwekiti, amacandelo ekiti angagcinwa kwiqondo lokushisa (15 - 25 ° C). Iirejenti zizinzile ukuya kuthi ga kunyaka omnye ukusuka kumhla wokwenziwa.
Sample Requirements
1. Isampulu esebenzayo: I-Swabs, i-plasma, i-serum kunye negazi elipheleleyo njl.
2. Ukugcinwa kwesampuli kunye nokuthutha: Isampuli kufuneka ihlolwe ngokukhawuleza
Materials kwaye Devices Required but Not Provided
1. Iiglavu ezingenamgubo ezilahlwayo
2. Isikhongozeli seBiohazard
3. Ipensile okanye nge
ProCedure
Oku kulandelayo kusebenzisa isampulu yesihlunu sokutsalwa kwe-swab lotion njengomzekelo ukuchaza ngokufutshane amanyathelo okusebenza kwe-reagent yotsalo kwisixhobo sokutsalwa kwe-nucleic acid yebhayoloji.Bioer NPA-32PokanyeSMUBUGCISA 32. Ngolunye uhlobo lwesampulu, nceda ubhekisele kwincwadana yomsebenzisi. Isenokusetyenziswa ngabathengi ngokomfuniselo wokufunyanwa:
1. I-Reagent Ukulungiselela
a. KubaYXN-VB03-32A-50A kwaye YXN-VB03-32A-100A
Yongeza i-500uL Lysis Buffer kwikholam 1 kunye ne-7 ye-2.2mL 96-deep-well plate, 500uL Wash Buffer I kwikholam 2 kunye ne-8, 500uL Wash Buffer II kwikholam 3, 4 kunye ne-9,10; I-70uL ye-Elution Buffer kwikholam yesi-5 kunye ne-11, i-180uL yamanzi acocekileyo kunye ne-20uL ye-MagaBio Reagent ukuya kwikholam yesi-6 kunye ne-12 (amaso amagnetic kufuneka axutywe kakuhle ngaphambi kokusetyenziswa),
b. KubaYXN-VB03-32A
Beka ii-reagents ezipakishwe kakuhle ezingama-96 kwiqondo lobushushu begumbi. Vuthulula ipleyiti enamanzi angama-96 ijonge phantsi izihlandlo ezithathu, kwaye uyikrazule ingxowa yeplastiki. I-Centrifuge i-reagent epakishwe ngaphambili kwimizuzwana embalwa (okanye ujiwule ngesandla amaxesha ambalwa) ukuphepha ukubambelela kwi-reagent eludongeni lweetyhubhu. Qhawula ifilimu yefoyile yealuminiyam yepleyiti yequla engama-96 kwaye uchonge icala lepleyiti (amaso azibuthe kukholomu #6 kunye #12),
2.Isampulu Ukutsalwa
1. Yongeza isampula ye-300uL kwiikholamu zeplate yequla engama-96 #1 kunye ne-#7, nceda uphephe ukungcoliseka,
2. Beka ipleyiti enzulu ye-96 kwisixhobo, faka iingcebiso ze-8-strip kwisixhobo,
3. Qhuba inkqubo ngokwezi nkqubo zilandelayo,
4. Emva kokuba ukucocwa okuzenzekelayo kuphelile, tshintshela i-elution Buffer kwikholamu 5 kunye ne-11 kwi-tube ecocekileyo ye-antinuclear 0.5mL centrifuge; ukuba awuyisebenzisi ngokukhawuleza, nceda uyigcine kwi-20 °C.
Performance Characteristics
1. Imveliso ekhutshiweyo ifunyenwe nge-high-sensitivity HBV DNA yokufumanisa i-reagent ukufikelela kwi-sensitivity ye-10 IU / mL. Imveliso ekhutshiweyo ifunyenwe yi-high-sensitivity HCV RNA yokufumanisa i-reagent ukufikelela kwi-sensitivity ye-50 IU / mL.
2. Khetha iisampuli ze-4 (i-serum / isampuli ye-plasma, isampuli ye-swab ye-nasopharyngeal, isampuli yeseli ye-cervical exfoliated), isampuli nganye ihlanjululwe ngamaxesha e-10 kunye ne-3 gradients (kubandakanywa nesampuli yasekuqaleni ye-concentrations ye-4), usebenzisa i-reagents efanelekayo kunye ne-agent yokuvavanya ukubona ngaphakathi. ijene yereferensi ngokwemiyalelo yemveliso, kwaye ixabiso le-Ct lebhetshi nganye liyohluka ngaphantsi kwe-1.
| Step | Kulungile Indawo | Program Igama | Wainto mnang Time(min:SS) | Mixnge Time(mkwi:SS) | Magnet Time(min:SS) | Aukuphelelwa ngamandla | Suchama | Volume ezintathu(μL) | Ti-emperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Biindlebe | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wuthuthu1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wuthuthu2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wuthuthu3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Uvavanyo | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Lahla | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. IGENERAL UKHUSELEKO.
Ukusebenzisa le mveliso ngendlela engachazwanga kumaxwebhu omsebenzisi kunokubangela ukulimala komntu okanye umonakalo kwisixhobo okanye isixhobo. Qinisekisa ukuba nabani na osebenzisa le mveliso ufumene imiyalelo kwiindlela zokhuseleko ngokubanzi kwiilabhoratri kunye neenkcukacha zokhuseleko ezinikwe kolu xwebhu.
1.1 Phambi kokusebenzisa isixhobo okanye isixhobo, funda kwaye uqonde ulwazi lokhuseleko olunikwe kumaxwebhu omsebenzisi anikwe ngumenzi wesixhobo okanye isixhobo.
1.2 Ngaphambi kokuphatha iikhemikhali, funda kwaye uqonde onke amaxwebhu eeNkcukacha zoKhuseleko (SDSs) asebenzayo kwaye usebenzise izixhobo ezifanelekileyo zokuzikhusela (iiglavu, iigawuni, ukukhuselwa kwamehlo, njl. njl.). Ukufumana ii-SDS, bona icandelo elithi “Amaxwebhu neNkxaso” kolu xwebhu.
2. Ikhemikhali ukhuseleko
UKUPHATHWA NGOKUBANZI KWEKHEMIKHI. Ukunciphisa iingozi, qinisekisa ukuba abasebenzi baselabhoratri bafunda kwaye basebenzise izikhokelo zokhuseleko jikelele zokusetyenziswa kweekhemikhali, ukugcinwa, kunye nenkunkuma ebonelelwe ngezantsi, kwaye uqhagamshelane ne-SDS efanelekileyo malunga nezilumkiso kunye nemiyalelo ethile: Funda kwaye uqonde i-Safety Data Sheets (SDSs) ebonelelwe yikhemikhali. umenzi phambi kokuba ugcine, uphathe, okanye usebenze ngazo naziphi na iikhemikhali okanye izinto eziyingozi.
2.1 Nciphisa ukudibana neekhemikhali. Nxiba izixhobo ezifanelekileyo zokuzikhusela xa uphethe imichiza (umzekelo, iindondo zokhuseleko, iiglavu, okanye iimpahla zokuzikhusela).
2.2 Ukunciphisa ukuphefumla kweekhemikhali. Musa ukushiya izikhongozeli zeekhemikhali zivuliwe. Sebenzisa kuphela umoya owaneleyo (umzekelo, i-fume hood).
2.3 Khangela rhoqo iikhemikhali ezivuzayo okanye ezichithekileyo. Ukuba kukho ukuvuza okanye ukuchitheka, landela iinkqubo zokucoca zomenzi njengoko kucetyiswa kwi-SDS.
2.4 Ukuphatha inkunkuma yeekhemikhali kwindawo yomlilo.
2.5 Ukuqinisekisa ukusetyenziswa kwezikhongozeli zenkunkuma eziphambili nezizisekondari. (Inkunkuma yokuqala
isikhongozeli sibamba inkunkuma ekhawulezileyo. Isikhongozeli sesibini sineendawo ezichithekileyo okanye ezivuzayo kwisikhongozeli sokuqala. Zombini izikhongozeli kufuneka zihambelane nenkunkuma kwaye zihlangabezane neemfuno zomdibaniso, zombuso, kunye nezengingqi zogcino lwesikhongozeli.).
2.6 Emva kokuchitha isikhongozeli senkunkuma, sitywine ngesivalo esisinikiweyo.
2.7 Faka uphawu (ngohlalutyo ukuba kuyimfuneko) inkunkuma eveliswa zizicelo ezithile, ii-reagents, kunye neesubstrates ezisetyenziswa kwilabhoratri yakho.
2.8 Ukuqinisekisa ukuba inkunkuma igciniwe, igqithiselwa, iyathuthwa, kwaye ilahlwe ngokwemigaqo yasekhaya, yephondo/yephondo kunye/okanye yesizwe.
2.9 Imathiriyeli ye-radioactive okanye ye-biohazardous inokufuna ukuphathwa ngokukodwa, kwaye imida yokulahla inokusebenza.
3.Ngebhayoloji ingozi ukhuseleko
I-Biohazard enokwenzeka. Ngokuxhomekeke kwiisampuli ezisetyenzisiweyo kwesi sixhobo, umphezulu unokuthathwa njenge-biohazard. Sebenzisa iindlela ezifanelekileyo zokucoca xa usebenza neengozi zebhayoloji.
I-BIOHAZARD. Iisampulu zebhayoloji ezifana nezicubu, ulwelo lomzimba, iiarhente ezosulelayo, kunye negazi labantu kunye nezinye izilwanyana zinamandla okusasaza izifo ezosulelayo. Landela yonke imimiselo esebenzayo yasekhaya, yelizwe/yephondo, kunye/okanye yesizwe. Nxiba izixhobo ezifanelekileyo zokukhusela, ezibandakanya kodwa zingaphelelanga koku: iimpahla zamehlo ezikhuselayo, isikhuselo sobuso, impahla/idyasi yelebhu, kunye neeglavu. Wonke umsebenzi kufuneka uqhutywe kwizibonelelo ezixhotyiswe ngokufanelekileyo kusetyenziswa izixhobo ezifanelekileyo zokhuseleko (umzekelo, izixhobo zokuthintela). Abantu kufuneka baqeqeshwe ngokwemigaqo esebenzayo kunye neemfuno zenkampani/iziko phambi kokuba basebenze ngemathiriyeli enokuthi yosuleleke.
Funda kwaye ulandele izikhokelo ezifanelekileyo kunye/okanye iimfuno zolawulo koku kulandelayo:
E-US: Izikhokelo zeSebe lezeMpilo kunye neeNkonzo zoLuntu zase-US ezipapashwe kwi-Biosafety kwi-Microbiological and Biomedical Laboratories efumaneka ku: www.cdc.gov/biosafety.
IMigangatho yoKhuseleko lweMisebenzi kunye neMpilo, iiPathogens ze-Bloodborne (29 CFR§1910.1030), zifunyenwe:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Iiprothokholi zeNkqubo yeBiosafety yenkampani/yeziko lakho lokusebenza kunye/nokuphatha izixhobo ezinokuthi zisuleleke. Ulwazi olongezelelekileyo malunga nezikhokelo ze-biohazard luyafumaneka: www.cdc.gov.
Kwi-EU: Jonga izikhokelo zengingqi kunye nomthetho malunga ne-biohazard kunye ne-biosafety precaution kwaye ubhekiselele kwezona ndlela zibalaseleyo zipapashwe kwi-World Health Organization (WHO) Laboratory Biosafety Manual, uhlelo lwesithathu, lufunyenwe: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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